Elisa Reader And Washer Principle

Readers need to have appropriate filter 650 nm and 450 nm.

Elisa reader and washer principle.

What an elisa reader does. The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically. The illustration below shows a workflow for a typical sandwich elisa assay. The elisa reader reads the plate.

The enzyme activity is measured using a substrate that changes color when modified by the enzyme. Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. Elisa is a plate based assay technique.

An immune reaction i e. The test is done in an elisa plate also known as a 96 well plate or microplate. Are available as fixed as well as adjustable volume as well as single channel and multi channel. And a plate reader software is used to plot standard curves and calculate results.

Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present.